Expression Analysis

The core facility provides expression analysis services for the Affymetrix and Illumina platforms. We have a long experience in expression analysis and can offer extensive data analysis support and bioinformatics.

Affymetrix Arrays

Affymetrix provide two generations of expression arrays, 3' (IVT) and 1.0 ST. 3' arrays were the first array type from Affymetrix, and are avaiable for a large number of species.

Illumina Arrays

The Illumina expression beadarray probes are bound to beads and randomly distributed onto arrays. Each array is individual and provided with a decoding sequence specifying the position of each probe. For the moment expression analysis is possible with human, mouse and rat arrays containing a very high feature redundancy. Multi sample formats provide effective handling and lower sample pricing allowing bigger experimental scale than other systems. The sample requirement for the Ambion amplification and labelling protocol that we use are approximately between 100ng -1µg (Vmax - 10ul) of pure total RNA.

Array Comparison

 3' (IVT) Gene ST Exon ST BeadArray
Platform AffymetrixAffymetrix Affymetrix Illumina
Coverage Whole-Genome Whole-Genome Whole-Genome Whole-Genome
Species Human, mouse, rat + many more Human, mouse, rat Human, mouse, rat Human, mouse, rat
Splicing Analysis No No Yes No
Minimum # Arrays 2-40 depending on array 2 2 12 / 16 / 24 depending on array

Probe Design Comparison

  •  Order Forms:
  •   Microarray Services
      Affymetrix Arrays (non-KI) 
      Illumina Arrays  
      Bioinformatics Order Form
  •  Price Lists:
  •   Affymetrix Arrays 
      Affymetrix Service Fees 
      Illumina Prices & Service Fees 
      qRT-PCR Prices & Serv. Fees 
  •  Resources: