Expression Analysis
The core facility provides expression analysis services for the Affymetrix and Illumina platforms. We have a long experience in expression analysis and can offer extensive data analysis support and bioinformatics.
Affymetrix Arrays
Affymetrix provide two generations of expression arrays, 3' (IVT) and 1.0 ST. 3' arrays were the first array type from Affymetrix, and are avaiable for a large number of species.
Illumina Arrays
The Illumina expression beadarray probes are bound to beads and randomly distributed onto arrays. Each array is individual and provided with a decoding sequence specifying the position of each probe. For the moment expression analysis is possible with human, mouse and rat arrays containing a very high feature redundancy. Multi sample formats provide effective handling and lower sample pricing allowing bigger experimental scale than other systems. The sample requirement for the Ambion amplification and labelling protocol that we use are approximately between 100ng -1µg (Vmax - 10ul) of pure total RNA.
Array Comparison
| 3' (IVT) | Gene ST | Exon ST | BeadArray | |
| Platform | Affymetrix | Affymetrix | Affymetrix | Illumina |
| Coverage | Whole-Genome | Whole-Genome | Whole-Genome | Whole-Genome |
| Species | Human, mouse, rat + many more | Human, mouse, rat | Human, mouse, rat | Human, mouse, rat |
| Splicing Analysis | No | No | Yes | No |
| Minimum # Arrays | 2-40 depending on array | 2 | 2 | 12 / 16 / 24 depending on array |
Probe Design Comparison








