Sample Requirements

Below are the sample requirement for expression microarray applications.

Please contact the core facility for other applications.

Expression Applications

PlatformArray TypeProtocolSample Fixed FormatStarting MaterialVolume
Affymetrix3'IVT ExpressNA50-500ng3ul
- " -Gene ST 1.0Ambion WTNA100-500ng3ul
- " -Gene ST 1.1- " -16/24/96100-500ng3ul
- " -Exon ST 1.0- " -NA200-500ng3ul
- " -3' / Gene ST 1.0NuGenNA500pg-50ng3ul
- " -miRNAFlashTagNA100ng-1ug3ul
AgilentSurePrint G3 ExpressionLow Input QuickAmp1/2/4/810-200ng1.5ul
- " -SurePrint G3 miRNAmiRNA Complete8100ng-1ug2ul
IlluminaHuman HT12Ambion Illumina TotalPrep1250-500ng8ul
- " -WG6- " -650-500ng8ul
- " -Ref8- " -850-500ng8ul
ABITLDASuperscript IIINA800ng/card8ul

Minimum sample requirement for array amplification and labeling. More material needed for concentration measurement and quality control.

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